http://tech.groups.yahoo.com/group/lrig-discussion/ LRIG Discussion Group is for comments or questions about Laboratory Robotics, or any Laboratory Automation topic. Thu, 05 Nov 2009 15:04:09 GMT ixi_steffen http://tech.groups.yahoo.com/group/lrig-discussion/message/6500 http://tech.groups.yahoo.com/group/lrig-discussion/message/6500 Dear Colleagues Does anyone have experiences with the lab on the chip technology and/or e.g. Raindance? Who is using that in reality for routinely cellular Tue, 03 Nov 2009 23:38:43 GMT jliscouski http://tech.groups.yahoo.com/group/lrig-discussion/message/6499 http://tech.groups.yahoo.com/group/lrig-discussion/message/6499 We've completed the third course in the initial portion of our program. The courses currently available are: 1) NEW -- Introduction to Laboratory Automation 2) Tue, 03 Nov 2009 19:24:24 GMT Rasmussen, Lynn http://tech.groups.yahoo.com/group/lrig-discussion/message/6498 http://tech.groups.yahoo.com/group/lrig-discussion/message/6498 I am going to recommend acoustic dispensing anyway. By the time you purchase a conventional liquid handler and pay for tips, in a few years you have the same Tue, 03 Nov 2009 16:59:45 GMT Neil Benn http://tech.groups.yahoo.com/group/lrig-discussion/message/6497 http://tech.groups.yahoo.com/group/lrig-discussion/message/6497 Hello, In general you should probably ask people to contact you directly with their recommendations on specific pieces of kit - I'll send you a private email Tue, 03 Nov 2009 16:21:39 GMT jonathansrosenblum http://tech.groups.yahoo.com/group/lrig-discussion/message/6496 http://tech.groups.yahoo.com/group/lrig-discussion/message/6496 We've worked pretty hard to try to implement some of the suggestions posted here?thanks for all of them! But I have a followup? Of those who have made Sat, 31 Oct 2009 14:44:17 GMT Bordeau, Brian http://tech.groups.yahoo.com/group/lrig-discussion/message/6495 http://tech.groups.yahoo.com/group/lrig-discussion/message/6495 Hi Jonathan, You may want to look into the Pocket Tip from Thermo. http://www.matrixtechcorp.com/downloads/PocketTips_Spec_Sheet_FINAL.pdf I've used them for a Fri, 30 Oct 2009 05:32:36 GMT LRIG Reader http://tech.groups.yahoo.com/group/lrig-discussion/message/6494 http://tech.groups.yahoo.com/group/lrig-discussion/message/6494 You should look into an ECHO...ECHo..ECho..Echo..echo........ Ima Bantoot President Foundation of Automation and Research Technologies ... [Non-text portions Thu, 29 Oct 2009 15:55:45 GMT Rasmussen, Lynn http://tech.groups.yahoo.com/group/lrig-discussion/message/6493 http://tech.groups.yahoo.com/group/lrig-discussion/message/6493 As you have discovered, what you describe is difficult to do. The first issue is that most 384-disposable tip instruments are not accurate at 0.5ul. The next Thu, 29 Oct 2009 15:53:11 GMT Kevin K. Olsen http://tech.groups.yahoo.com/group/lrig-discussion/message/6492 http://tech.groups.yahoo.com/group/lrig-discussion/message/6492 Jonathan, The key to dispensing small volumes of DMSO to multiple plates is to fill the tip completely, then dispense a small volume back into the source Thu, 29 Oct 2009 15:52:13 GMT robin384 http://tech.groups.yahoo.com/group/lrig-discussion/message/6491 http://tech.groups.yahoo.com/group/lrig-discussion/message/6491 Hi Dry dispensing 0.5ul is at the limit of most air displacement systems, however try this as it should work with the lower volume tips. Just submerge the tips Thu, 29 Oct 2009 15:52:09 GMT James Batchelor http://tech.groups.yahoo.com/group/lrig-discussion/message/6490 http://tech.groups.yahoo.com/group/lrig-discussion/message/6490 If they are all copies, you're best bet is to do the following 0.5uL air 5uL mother plate asiprate dispense back to the mother 1uL (leaving 4 in the tips)..may Thu, 29 Oct 2009 15:52:08 GMT Neil Benn http://tech.groups.yahoo.com/group/lrig-discussion/message/6489 http://tech.groups.yahoo.com/group/lrig-discussion/message/6489 Hello, 0.5ul is a challenge to dispense with a typical air displacement system (despite what vendors may say!). Try this: * Aspirate 3ul of sample in DMSO * Thu, 29 Oct 2009 15:51:39 GMT Szoke, Balazs http://tech.groups.yahoo.com/group/lrig-discussion/message/6488 http://tech.groups.yahoo.com/group/lrig-discussion/message/6488 Jonathan, Aspirate an airgap (couple of uL's) and then 3.5 - 4 uL compound/DMSO from your source plate. Dispense back 0.5 - 1.0 uL to your source well (in Thu, 29 Oct 2009 03:12:51 GMT jonathansrosenblum http://tech.groups.yahoo.com/group/lrig-discussion/message/6487 http://tech.groups.yahoo.com/group/lrig-discussion/message/6487 I'm working through a protocol to dry dispense a set of assay ready plates. All the compounds are in DMSO. I'd like to dispense 5 assay-ready plates from a Wed, 21 Oct 2009 14:07:09 GMT kek01 http://tech.groups.yahoo.com/group/lrig-discussion/message/6486 http://tech.groups.yahoo.com/group/lrig-discussion/message/6486 Hi, thanks for the info on the Janus. . .we have those but I haven't looked at what can be done with those yet . .encouraging though from what you've posted.