http://tech.groups.yahoo.com/group/microarray/ micro & macro arrays Wed, 04 Nov 2009 09:04:00 GMT Neil Graham http://tech.groups.yahoo.com/group/microarray/message/19890 http://tech.groups.yahoo.com/group/microarray/message/19890 Dear Muhammad, The experimental design looks fine and having 3 biological should be ok. With the 1 time point issue, I would recommend making sure that the Wed, 04 Nov 2009 09:03:56 GMT Luigi Marchionni http://tech.groups.yahoo.com/group/microarray/message/19889 http://tech.groups.yahoo.com/group/microarray/message/19889 you need to specify, platform, color scheme, and the like, otherwise it will be impossible to answer. Luigi Marchionni ... [Non-text portions of this message Wed, 04 Nov 2009 09:03:13 GMT sadia saeed http://tech.groups.yahoo.com/group/microarray/message/19888 http://tech.groups.yahoo.com/group/microarray/message/19888 Hello Javid, I am sorry, i cannot comment on your design as it is not my field. But nice to see a muslim guy here. Regards, Sadia ... From: javid_mirza Tue, 03 Nov 2009 14:58:28 GMT Agnieszka Lichanska http://tech.groups.yahoo.com/group/microarray/message/19887 http://tech.groups.yahoo.com/group/microarray/message/19887 Hi Muhammad, single time point has to be compared to some sort of baseline, untreated plants/leaves will be the most obvious choice as a baseline. Also an Tue, 03 Nov 2009 14:57:46 GMT crosbyseth http://tech.groups.yahoo.com/group/microarray/message/19886 http://tech.groups.yahoo.com/group/microarray/message/19886 Hello Muhammad, Statistically speaking, the experiment is fine as long as it addresses your hypothesis. You could possibly find, after looking at the data, Mon, 02 Nov 2009 12:23:56 GMT Chris Seidel http://tech.groups.yahoo.com/group/microarray/message/19885 http://tech.groups.yahoo.com/group/microarray/message/19885 Hi Mervi, ... The fold-change, and the scale used to plot changes are usually different things. When people discuss fold-change, they do so in linear space, so Mon, 02 Nov 2009 12:19:11 GMT javid_mirza http://tech.groups.yahoo.com/group/microarray/message/19884 http://tech.groups.yahoo.com/group/microarray/message/19884 Hi Fellows, I'm starting RNA extractions from stressed leaves for microarray work with following parameters in mind Genotype= 1 Time point= 1 (4 weeks after Fri, 30 Oct 2009 09:14:34 GMT Manoj http://tech.groups.yahoo.com/group/microarray/message/19883 http://tech.groups.yahoo.com/group/microarray/message/19883 I thought of passing this around to see if there are any such needs at your lab. Please feel free to pass this around to others whom you think might benefit Mon, 26 Oct 2009 09:34:43 GMT genequantification http://tech.groups.yahoo.com/group/microarray/message/19882 http://tech.groups.yahoo.com/group/microarray/message/19882 International qPCR 2010 Symposium & Exhibition in Vienna 7th ? 9th April 2010 The focus of the qPCR 2010 Event will be "The ongoing evolution of qPCR" Our Mon, 26 Oct 2009 08:40:41 GMT Seth Crosby http://tech.groups.yahoo.com/group/microarray/message/19881 http://tech.groups.yahoo.com/group/microarray/message/19881 Illumina claims with the HT-12 you can believe down to a 1.3-fold change with a p-value of 0.01. The reason we log transform (and, by the way, it is usually Mon, 26 Oct 2009 08:40:03 GMT Ian McFarlane http://tech.groups.yahoo.com/group/microarray/message/19880 http://tech.groups.yahoo.com/group/microarray/message/19880 Hi, We have three open positions in Cambridge (UK) for lab staff to run our complement of 5 next-gen sequencers (3xSOLiD, 1x454, 1xIllumina). The lab also Fri, 23 Oct 2009 17:26:40 GMT smurffi03 http://tech.groups.yahoo.com/group/microarray/message/19879 http://tech.groups.yahoo.com/group/microarray/message/19879 Dear All, I'm analysing a pilot study with Illumina HT-12 data. I want to find differentially expressed genes but I'm struggling with the interpretation of Fri, 09 Oct 2009 07:35:21 GMT Eric Antoniou http://tech.groups.yahoo.com/group/microarray/message/19878 http://tech.groups.yahoo.com/group/microarray/message/19878 Dear Colleagues The Jackson Laboratory gene expression and Next Generation Sequencing core laboratory is doing a survey of comparable core labs to benchmark Mon, 05 Oct 2009 07:30:16 GMT crosbyseth http://tech.groups.yahoo.com/group/microarray/message/19877 http://tech.groups.yahoo.com/group/microarray/message/19877 There are many platforms and many arrays which already exist which you could buy tomorrow. What species? Fri, 02 Oct 2009 08:35:03 GMT saifmunshi http://tech.groups.yahoo.com/group/microarray/message/19876 http://tech.groups.yahoo.com/group/microarray/message/19876 Hi All We are planning to buy a real Time PCR within 20000 USD which should have at least 96 wells . We prefer ABI, Bio Rad . IS it possible ?? -Saif