http://tech.groups.yahoo.com/group/molecular-biology-notebook/ Molecular Biology Notebook Tue, 05 Jan 2010 23:27:41 GMT Tiznado, Martin http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8532 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8532 Adrian, try to use the solution in which the bacteria was growing instead of the bacteria alive. You can sterilize the solution using filters, Martin 2010/1/3 Tue, 05 Jan 2010 07:32:12 GMT believe_adrian http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8531 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8531 Dear All, I tried to perform hemagglutination by using pseudomonas aeruginosa with human blood. However I did not succeed in doing it. Any ideas? I collect the Mon, 04 Jan 2010 07:38:37 GMT loyolite112000 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8530 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8530 This year's Nobel Prize in Chemistry awards Venkatraman Ramakrishnan, Thomas A. Steitz and Ada E. Yonath for having showed what the ribosome looks like and how Sat, 02 Jan 2010 11:05:41 GMT Tang TH http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8529 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8529 can u tell more, what do u mean by normalised cDNA library....? Tang Thean Hock (Dr.rer.nat, Germany) Advanced Medical & Dental Institute (AMDI) Universiti Wed, 30 Dec 2009 15:58:35 GMT banya http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8528 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8528 hii there i am working on an ectoparasite of fish and want to study the immune gene expression in response to this infection....could someone help me with Wed, 23 Dec 2009 16:37:20 GMT satrupa_mishra06 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8527 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8527 hi there i am here stuck with the protein extraction protocol.. can anyone help me with a standard method for extraction of protein from plant tissue. i want Wed, 23 Dec 2009 16:35:32 GMT genequantification http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8526 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8526 qPCR NEWS - free REST 2009 version Dear qPCR NEWS Reader, Best Wishes for the Holiday Season and a Happy and Successful New Year 2010 Our newsletter informs Tue, 22 Dec 2009 09:59:50 GMT loyolite112000 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8525 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8525 Researchers at Newcastle University in England report they have coaxed the first human sperm cells from embryonic stem cells, in a remarkable demonstration of Sat, 12 Dec 2009 17:34:34 GMT zia_dna http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8524 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8524 my name is zia.i m working in moleculer diagnostic laboratory in lahore.i want to start detection of BCR-ABL gene responsible for chronic myeloid leukemia.i ve Fri, 11 Dec 2009 22:21:28 GMT ALI RABAAN http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8523 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8523 Hi, Dealing with viral load is a difficult thing. Try to convert the value into Log10 than compare the two results. It should not exceed + or - 0.5 Log between Fri, 11 Dec 2009 22:21:25 GMT abhijit rath http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8522 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8522 Hi Zia   What about your sample duplicates/triplicates Ct values in real time PCR?Are they consistent?   Abhijit ... From: kantharaj gowda Fri, 11 Dec 2009 07:50:40 GMT kantharaj gowda http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8521 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8521 Hi It depends upon the viral load in the patient, it varies, it is not uniform from patient to patient and duaration he had infection. ... From: zia_dna Wed, 09 Dec 2009 16:17:33 GMT zia_dna http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8520 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8520 my name is zia,i m working in a moleculer diagnostic laboratory.i do nested pcr for hcv genotyping and real time pcr for viral load determination.we use Qiagen Sun, 06 Dec 2009 08:26:27 GMT Asif raza http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8519 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8519 Yes, I agree with Khurrum Bashir, expression of same gene under 35S promoter may be  different in different transgenic lines. Even the expression might be Sun, 06 Dec 2009 05:09:43 GMT loyolite112000 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8518 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8518 Cells in the immune system like Macrophages and dendritic cells are the first line of defense in recognizing various kinds pathogens.These cells are developed