http://tech.groups.yahoo.com/group/molecular-biology-notebook/ Molecular Biology Notebook Sat, 07 Nov 2009 17:07:41 GMT kantharaj gowda http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8491 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8491 Hi If your protein in solution, store them in as small samples.you take one each when you require.  Once you thaw it, you can refreeze it perhaps once or Sat, 07 Nov 2009 06:19:00 GMT Asif raza http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8490 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8490 Dear friends, I have isolated protein from the samples of transgenic cotton carrying insecticidal genes with the purpose to quantify it. Meanwhile I have Mon, 02 Nov 2009 17:55:17 GMT mislaini herson http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8489 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8489 Hi rohit..is it possible the primer degaded.? .because previously I've got band for amplification but after that no band..... ... Dari: rohit cdri Mon, 02 Nov 2009 17:55:11 GMT mislaini herson http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8488 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8488 I extracted total RNA from cell culture and impurity 260/280= 2.03 ng/ul and 260/280=0.84 and data for cDNA from total RNA impurity 260/280=1.65 ng/ul and Mon, 02 Nov 2009 07:44:24 GMT rohit cdri http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8487 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8487 You can run primer on gel ( 1.2 to 1.5 % agarose) just to check single band ..... If your primer is degraded you will get smeared .......... Sat, 31 Oct 2009 22:37:49 GMT Walid AlZyoud http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8486 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8486 Dear Suzane, the primers will not appear as a sharp band, it will appear as a very faint band, so I think it does not worth to evaluate your primers by this Sat, 31 Oct 2009 21:27:49 GMT Aun http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8485 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8485 Hi guys, I am working on MTOR inhibitors in prostate cancer. It would be really great to know and collaborate with some one who is working on MTOR as well, Sat, 31 Oct 2009 21:12:01 GMT SUZAN RAGHEB http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8484 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8484 Hello all, can I run different primer concenrations  (without sample) on gel before  the begining of my work to evaluat the most suitable  or the  most Mon, 26 Oct 2009 01:38:48 GMT SUZAN RAGHEB http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8483 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8483 Hello all, dose the  measuring  of DNA concentration" with spectrophotometer " undergo using a law ? how can I use this law: DNA conc in microgram/ml =OD Sun, 25 Oct 2009 17:22:16 GMT genequantification http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8482 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8482 International qPCR 2010 Symposium & Exhibition in Vienna 7th ? 9th April 2010 The focus of the qPCR 2010 Event will be "The ongoing evolution of qPCR" Our Sun, 18 Oct 2009 05:00:23 GMT sajid ali http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8481 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8481 Dear friend   Hope you are fine and doing well   Below are the answers of your questions which are highlighted.   1. What must occur in binary fission Tue, 13 Oct 2009 04:13:23 GMT Sarah http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8480 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8480 I hate to be one of those who takes their homework questions and tries to pawn them off on the web, but a number of these questions are NOT clearly explained Mon, 12 Oct 2009 03:03:13 GMT sidjam http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8479 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8479 Please could you inform us about the primer used for second DNA strand synthesis in Reverse transcription. Oligo dT primes the first DNA strand synthesis. Sat, 10 Oct 2009 01:32:53 GMT elham rastegari http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8478 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8478 Dear Hamid, I appreciate your consideration. I would be gratful if you could answer these qThe recepie that i used to prepare sample buffer is Sample loading Sat, 10 Oct 2009 01:26:06 GMT Murat Kasap http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8477 http://tech.groups.yahoo.com/group/molecular-biology-notebook/message/8477 To make a reliable phylogenetic analysis 3 softwares are needed. 1. Clustal X 2. BioEdit 3. Phylip   With clustal X you need to align your sequences. With